Male Decapacitation Factor SPINK3 Blocks Membrane Hyperpolarization and Calcium Entry in Mouse Sperm
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چکیده
منابع مشابه
Membrane hyperpolarization during human sperm capacitation.
Sperm capacitation is a complex and indispensable physiological process that spermatozoa must undergo in order to acquire fertilization capability. Spermatozoa from several mammalian species, including mice, exhibit a capacitation-associated plasma membrane hyperpolarization, which is necessary for the acrosome reaction to occur. Despite its importance, this hyperpolarization event has not been...
متن کاملControl of the low voltage-activated calcium channel of mouse sperm by egg ZP3 and by membrane hyperpolarization during capacitation.
Sperm adhesion to egg zonae pellucidae initiates sperm acrosome reactions, an exocytotic event that is an early step during fertilization. Previously, it was suggested that zona pellucida-evoked Ca2+ entry into sperm through low voltage-activated Ca2+ channels is an essential step in acrosome reactions, based on the inhibitory effects of Ca2+ channel antagonists. However, analysis of this chann...
متن کاملDecapacitation factor purification with lipid solvents.
In 1957, Chang reported the presence of a substance in the seminal plasma of several mammalian species which had the ability to reverse the capacitation process (decapacitation factor or DF). Bedford & Chang (1962) were partially able to purify DF by ultra-centrifugation. Since that time, a great deal of effort has been given to the purification, isolation and identification of this substance (...
متن کاملCalcium Clearance Mechanisms of Mouse Sperm
The spermatozoon is specialized for a single vital role in fertilization. Past studies show that Ca2+ signals produced by the opening of plasma membrane entry channels initiate several events required for the sperm to reach and enter the egg but reveal little about how resting [Ca2+]i is maintained or restored after elevation. We examined these homeostatic mechanisms by monitoring the kinetics ...
متن کاملthe effect of curcumin on intracellular ph (phi), membrane hyperpolarization and sperm motility
background: curcumin has shown to affect sperm motility and function in vitro and fertility in vivo. the molecular mechanism(s) by which curcumin affects sperm motility has not been delineated. since modulation of intracellular ph (phi) and plasma membrane polarization is involved in sperm motility, the present study was conducted to investigate the effect of curcumin on these sperm (human and ...
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ژورنال
عنوان ژورنال: Frontiers in Cell and Developmental Biology
سال: 2020
ISSN: 2296-634X
DOI: 10.3389/fcell.2020.575126